TARGETED METABOLOMICS
Amino Acids Targeted Panel
At Metabolon, we understand the crucial role amino acids play in physiological processes, and we’ve established best-in-class expertise for their detection. This panel focuses on specific amino acids and their metabolic pathways and can be used to track biomarkers and enhance biological understanding across preclinical and clinical research.
About Amino Acids
Amino Acids (AA) are the foundational building blocksfor peptides and proteins. These small molecules regulate metabolic pathways that are involved in cell maintenance, growth, reproduction, and immunity. Branched chain amino acids play a large role in building muscle tissue and participate in increasing protein synthesis. Amino acids also play a role in cell signaling, gene expression and protein phosphorylation. Maintaining an optimal balance of amino acids is vital to maintaining a stable equilibrium of physiogolical processes.
Panel Applications
The amino acid targeted panel measures free amino acids in biofluids, cells and tissues and can be used in many different applications including the following:
- Metabolic disorders
- Human and animal nutrition
- Bioprocessing
- Drug development
About Amino Acids
| LLOQ* | |
|---|---|
| Metabolite | Plasma/Serum (µg/mL) |
| Alanine | 1.00 |
| Arginine | 1.00 |
| Asparagine | 0.250 |
| Aspartic Acid | 0.250 |
| Glutamic Acid | 0.500 |
| Glutamine | 2.50 |
| Glycine | 0.750 |
| Histidine | 0.500 |
| Isoleucine | 0.250 |
| Leucine | 0.500 |
| Lysine | 1.00 |
| Methionine | 0.125 |
| Phenylalanine | 0.400 |
| Proline | 1.00 |
| Serine | 0.400 |
| Threonine | 0.500 |
| Tryptophan | 0.500 |
| Tyrosine | 1.00 |
| Valine | 1.00 |
| Trans-4-hydroxyproline | 0.0500 |
| Ornithine | 0.250 |
| Citruline | 0.200 |
*Lower Limit of Quantitation (LLOQ) varies for each sample type.
Analysis Method and Instrumentation
LC-MS/MS (Agilent 1290 UHPLC/Sciex QTrap 5500)
| Sample Type and Required Amounts | |
|---|---|
| Sample Type | Sample Requirements |
| Plasma/Serum | 100 to 150 µL |
Others on request
- N25 years of experience with over 15,000 projects completed
- N45+ PhDs on staff
- N5,400+ metabolites in our library
- NWe continuosly partner with global cohorts and medical centers of excellence to reveal promising new biomarkers
- NPublished in over 4,000+ publications
